Clark T. Hung

Professor of Biomedical Engineering
Chair of Undergraduate Studies

351 Engineering Terrace
1210 Amsterdam Avenue, Mail Code: 8904
New York , NY 10027

Phone: +1 212-854-6542
Fax: +1 212-854-8725
Email: cth6@columbia.edu
CV

Research Areas:

Effects of physical, mechanical, and chemical stimuli on musculoskeletal cells related to cellular and tissue engineering.

Cellular Engineering Laboratory

Education

  • 1990: Sc.B., Biomedical Engineering, Brown University
  • 1992: M.S.E., Bioengineering, University of Pennsylvania
  • 1995: Ph.D., Bioengineering, University of Pennsylvania

 

Awards/ Honors/ Professional Experience (Selected)

  • 1990-95: NIH Doctoral Trainee on Bone and Cartilage Biology
  • 1995: Instructor, Department of Bioengineering, University of Pennsylvania
  • 1996: Solomon Pollack Award for Graduate Research
  • 1997: Whitaker Special Opportunity Award Postdoctoral Fellowship (Center for Biomedical Engineering, Columbia University)
  • 1997: Assistant Professor of Biomedical Engineering, Columbia University
  • 1998-present: Director, "Physical Effects on Cells: Biomedical Engineering" Course offered by the Columbia University Summer High School Program
  • 2000: Guest editor, Cell & Tissue Engineering issue of Journal of Biomechanical Engineering (June issue)
  • 2002: Kim Award for Faculty Involvement
  • 2002: Associate Professor of Biomedical Engineering (untenured), Columbia University
  • 2003: Negma-Lerards Prize, 3rd International Symposium on Mechanobiology of Cartilage and Chondrocyte, Brussels, Belgium, May 16-17
  • 2004: Associate Professor of Biomedical Engineering (tenured), Columbia University
  • 2004: Co-author, The John Paul Stapp Best Paper Award in the 2003 Stapp Car Crash Journal presented at the 47th Stapp Car Crash Conference. A tissue level tolerance criterion for living brain developed with an in vitro model of traumatic mechanical loading(Morrison III, B lead author)
  • 2006-present: Associate Editor: Journal of Biomechanical Engineering
  • 2008-present: Deputy Editor: Journal of Orthopaedic Research
  • 2009-present: Editor: Journal of Orthopaedic Research & Reviews (Dove Press, open access)
  • 2009: Fellow, American Institute for Medical & Biological Engineering (AIMBE)
  • 2010: Fellow, American Society of Mechanical Engineers (ASME)

 

ACTIVE GRANT SUPPORT

1. Co-Principal Investigator: NIH 1R01AR060361-01 (Ateshian GA, Contact PI): Optimizing nutrient supply in large engineered cartilage tissue constructs ($225,000 yearly DC: $1,742,366), 10% yearly effort- 9/20/10-8/31/15. Description: This grant examines the optimization and use of nutrient channels to cultivate large clinically relevant cartilage grafts.

2. Co-Principal Investigator (contact PI: Kaplan, David with Levin, Michael and Vunjak-Novakovic, Gordana): NIH 1R01AR061988-01: Electrotherapeutic Strategies for Connective Tissue Repair ($3,650,242total costs).  7.5% yearly effort- 7/1/2011-6/30/2016. Description: Three specific aims will be pursued: (a) Biophysical regulation of chondrogenesis and osteogenesis in adult human stem cells, (b) Electrotherapeutic bioreactormodels for regeneration of cartilage/bone tissues, and (c) Animal studies of cartilage/bone regeneration. The main technological impact will be the development of a new generation of electrotherapeutic medical devices termed BioDomes.

3. Co-Principal Investigator (with Bulinski, JC): CDMRP: OR130124 IDEA: Electric field stimulation enhances healing of post-traumatic osteoarthritic cartilage.$500,000 direct costs (TC: $643,989), 10/1/14-9/30/17. Description: The proposal will explore the application of electric fields to promote healing of cartilage lesions by inducing migration of synovial derived stem cells.  In vitro studies as well as in vivo studies in a canine cartilage defect model are planned with collaborators at Brown University (Aaron RK) and University of Missouri (Cook JL) (8% effort)

4. Co-Principal Investigator (with Brown, LM): NYSTEM: N13S-005: Large-scale biochemical profiling for stem cell research in New York (TC:$1,624,453), 4/1/14-3/31/18. The proposal seeks funds to purchase acquire a new liquid chromatograph-mass spectrometer and increase staffing level to support this equipment to support metabolomics and proteomics studies pertaining to stem cell research at Columbia and for other New York state researchers.

5. Investigator: NIH: NIBIB 5P41EB002520:Core- Tissue Engineering Bioreactor (Vunjak-Novakovic, PI): 4% yearly effort, 9/1/09-8/31/19.  Description: The focus of this core will be on the development and utilization of novel bioreactors designed to precisely control the cellular microenvironment, impart multiple physical stimuli, and enable real time imaging of cells and tissues at various hierarchical scales. Osteochondral (cartilage/bone) tissues and myocardium are selected as paradigms of distinctly different, clinically relevant engineered tissues to serve as models for bioreactor development and validation.

6. Engineering PI: Coulter Foundation/Columbia CTV WHCF CU12-0369 (with Clinical PI: Cook JL): ECHON- Engineered Osteochondral Graft for Joint Repair, 9/1/14-8/31/15 (TC:$156,478). Description: The project is joint between the Coulter Translational Research Program of the University of Missouri and Columbia University.  The project aims to translate proprietary osteochondral graft technology to human cells in culture and in vivo studies.

7. Co-Investigator (PI: Lu, HH)N13G-107NYSTEMIDEA proposal: Stem Cell-mediated integrative Cartilage Repair, total requested $330,000 (yr 1 total 152,073), 6/1/14-5/31/16 (5% effort).  The proposal seeks to develop an electrospun, nano-fibrous polymer interface to facilitate osteochondral graft integration with the host tissue.

8. Principal Investigator: MTF Investigator Grant: Local Dexamethasone Delivery for Osteochondral Grafting, TC: $300,000, 2/1/15-1/31/18.  This application aims to improve osteochondral repair of full thickness cartilage defects by intra-articular delivery of the steroid dexamethasone from engineered osteochondral plugs placed in the graft donor sites as part of an autologous osteochondral transfer procedure.  The team includes collaborators from Columbia (Ateshian),  the University of Pittsburgh (Marra) and University of Missouri (Cook, Kuroki).  (paperwork pending)

Research Summary

Dr.Hung has been pursuing in-depth multidisciplinary collaborations with faculties and students from the Departments of Biological Sciences, Mechanical Engineering, Chemical Engineering and Orthopaedic Surgery using with state-of-the-art biological and engineering tools to perform research aimed atthe study of physical effects (e.g., cell deformation, fluid flow effects,hydrostatic pressure) on cells and tissues, and the incorporation of these forces in strategies to develop functional tissue substitutes of clinical relevance. An understanding of the effects of physical forces on cells isimportant in the development of effective tissue replacements which mimic orrestore normal tissue structure-function in orthopaedic and other load-bearing tissues of the body. Such studies are aim at alleviating the most prevalent and chronic problems afflicting the musculoskeletal system such as arthritis, and problems related to sports and occupational injuries.

 

Selected Publications

  1. Hung, CT, Pollack SR, Reilly TM, Brighton CT: Real-time calcium response of cultured bone cells to fluid flow. Clin Orthop Rel Res  313:256-269, 1995.
  2. Hung, CT, F.D. Allen, S.R. Pollack, E.T. Attia, J.A. Hannafin, and P.A. Torzilli, Intracellular calcium response of ACL and MCL ligament fibroblasts to fluid-induced shear stress. Cell. Signal., 1997. 9(8): p. 587-594.
  3. Mauck RL, Soltz MA, Wang CC-B, Wong DD, Chao P-HG, Valhmu WB, Hung, CT, Ateshian GA: Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J Biomech Eng 122:252-260, 2000.
  4. Hung, C., D. Henshaw, C. Wang, R. Mauck, F. Raia, G. Palmer, V. Mow, A. Ratcliffe, and W. Valhmu,Mitogen-activated protein kinase signaling in bovine articular chondrocytes in response to fluid flow does not require calcium mobilization. J Biomechanics, 2000. 33: p. 73-80.
  5. Lima, EG, Tan AR, Tai T, Bian L, Ateshian GA, Cook JL, and Hung CT, Physiologic deformational loading does not counteract the catabolic effects of interleukin-1 in long-term culture of chondrocyte-seeded agarose constructs. J Biomech, 2008. 41(15): p. 3253-3259.
  6. Lima EG, Tan AT, Tai T, Bian L, Stoker AM, Ateshian GA, Cook JL, and Hung CT, Differences in Interleukin-1 Response between Engineered and Native Cartilage. Tissue Eng Part A, 2008. 14(10): p. 1721-1730.
  7. Ng KW, Lima EG, Bian L, O’Conor CJ, Jayabalan PS, Stoker AM, Kuroki K, Cook CR, Ateshian GA, Cook JL, Hung CT: Passaged adult chondrocytes can form engineered cartilage with functional mechanical properties: A canine model. Tissue Engineering A 16(3):1041-1051, 2010.
  8. Bian L, Stoker AM, Marberry KM, Ateshian GA, Cook JL, Hung CT: Effects of Dexamethasone on the Functional Properties of Cartilage Explants during Long-term Culture.  Am J Sports Med 38(1):78-85, 2010.
  9. Sampat SR, O’Connell GD, Fong JV, Aguaron EA, Ateshian GA, Hung CT. Synovium derived stem cells for cartilage tissue engineering. Tissue Eng Part A 17(17-18):2259-2265, 2011.
  10. Oswald ES, Brown LM, Bulinski JC, Hung CT: Label-free protein profiling of adipose-derived human stem cells under hyperosmotic treatment. J Proteome Res 10(7):3050-3059, 2011.
  11. Sampat SR, Dermksian MV, Oungoulian SR, Winchester RJ, Bulinski JC, Ateshian GA, and Hung CT, Applied osmotic loading for promoting development of engineered cartilage. J Biomechanics, 46:2674-81, 2013.
  12. Alegre-Aguaron E, Sampat SR, Xiong JC, Colligan RM, Bulinski JC, Cook JL, Ateshian GA, Brown LM, and Hung CT, Growth factor priming differentially modulates components of the extracellular matrix proteome in chondrocytes and synovium-derived stem cells. Plos One, 2014. 9(2): p. e88053.
  13. Cigan A,D Nims RJ, Albro MB, Vunjak-Novakovic G, Hung CT, Ateshian GA. Nutrient Channels and Stirring Enhanced the Composition and Stiffness of Large Cartilage Constructs, J Biomechanics, 47(16):3847-54, 2014.
  14. Tan AR, Alegre-Aguaron E, O’Connell GD, VandenBerg CD, Aaron RK, Vunjak-Novakovic G, Bulinski JC, Ateshian GA, Hung CT, Passage-Dependent Relationship between Mesenchymal Stem Cell Mobilization and Chondrogenic Potential, Osteoarthritis Cartilage, 23(2):319-27, 2015.
  15. O’Connell GD, Tan AR, Cui V, Bulinski JC, Cook JL, Attur M, Abramson SB, Ateshian GA, Hung CT: Human chondrocyte migration behavior to guide development of engineered cartilage. J Tissue Eng Reg Med, 2014, in press.

Courses Taught

  • BMEN 4501, 4502: Tissue Engineering I and II
  • BMEN 3820: Biomedical Engineering Laboratory II
  • Biomedical Engineering: Physical Effects on Cells (Summer High School Course in July)

Effects of physical, mechanical, and chemical stimuli on musculoskeletal cells related to cellular and tissue engineering.


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