Education

• 1990: Sc.B., Biomedical Engineering, Brown University
• 1992: M.S.E., Bioengineering, University of Pennsylvania
• 1995: Ph.D., Bioengineering, University of Pennsylvania

Awards/ Honors/ Professional Experience (Selected)

• 1990-95: NIH Doctoral Trainee on Bone and Cartilage Biology
• 1995: Instructor, Department of Bioengineering, University of Pennsylvania
• 1996: Solomon Pollack Award for Graduate Research
• 1997: Whitaker Special Opportunity Award Postdoctoral Fellowship (Center for Biomedical Engineering, Columbia University)
• 1997: Assistant Professor of Biomedical Engineering, Columbia University
• 1998-present: Director, "Physical Effects on Cells: Biomedical Engineering" Course offered by the Columbia University Summer High School Program
• 2000: Guest editor, Cell & Tissue Engineering issue of Journal of Biomechanical Engineering (June issue)
• 2002: Kim Award for Faculty Involvement
• 2002: Associate Professor of Biomedical Engineering (untenured), Columbia University
• 2003: Negma-Lerards Prize, 3rd International Symposium on Mechanobiology of Cartilage and Chondrocyte, Brussels, Belgium, May 16-17
• 2004: Associate Professor of Biomedical Engineering (tenured), Columbia University
• 2004: Co-author, The John Paul Stapp Best Paper Award in the 2003 Stapp Car Crash Journal presented at the 47th Stapp Car Crash Conference. A tissue level tolerance criterion for living brain developed with an in vitro model of traumatic mechanical loading(Morrison III, B lead author)
• 2006-present: Associate Editor: Journal of Biomechanical Engineering
• 2008-present: Deputy Editor: Journal of Orthopaedic Research
• 2009-present: Editor: Journal of Orthopaedic Research & Reviews (Dove Press, open access)
• 2009: Fellow, American Institute for Medical & Biological Engineering (AIMBE)
• 2010: Fellow, American Society of Mechanical Engineers (ASME)

ACTIVE GRANT SUPPORT

Principal Investigator:NIH: NIAMS 1R01AR052871-04: Chondrocyte mechanotransduction usingmicrofluidics; 7/1/06-6/30/10. Description: This grant will study thedynamic osmotic loading response of cultured chondrocytes using a custommicrofluidics device, epifluorescence microscopy and cell biology techniques tomonitor cytoskeleton rearrangement and gene expression.(No cost extension)

Principal Investigator:NIH: NIAMS 1R01AR46568-10: Physiologic loading for cartilagetissue engineering; 9/1/09-8/31/13. This grant (a competing continuation)investigates the use of applied physiologic loading in a custom bioreactor togrow functional articular cartilage followed by implantation in acanine kneedefect model. Development of a theoretical model to predict cracking inengineered constructs subjected to loading in a defect site.

Principal Investigator:NIHAR46568-10S1 BIRT Supplement (7/1/10-6/30/11): Thisproposal aims to include proteomics as a screening tool for optimization ofcellpassaging/priming for functional tissue engineering of cartilage.

Co-Principal Investigator:NIH 1R01AR060361-01(Ateshian GA, Contact PI): Optimizing nutrientsupply in large engineered cartilage tissue constructs, 9/20/10-8/31/15.Thisgrant examines the optimization and use of nutrient channels (0.5 mm diameter)to cultivate large clinically relevant cartilage grafts.

Principal Investigator:NIH 1S10RR027943-01:Columbia AFM Equipment Grant(4/1/10-3/31/11). This proposal requests funds for a Veeco BioScope Catalystatomic force microscope (AFM) system mounted on an inverted Olympus IX81microscope equipped with a FluoView FV1000 laser scanning confocal microscopysystem (LSCM).

Co-Investigator:NIH R21 EB11869-01A1: SilkHydrogel for Functional Cartilage Tissue Engineering (PI: Vunjak-NovakovicG): 3/1/10-2/28/12. Description: This grant investigates the use of novel silkhydrogel scaffolds for cartilage tissue engineering. Different silkformulations will be characterized in terms of solute diffusivity and hydraulicpermeability and correlated with functional development of engineered cartilagetissues in culture.

Research Summary

Dr.Hung has been pursuing in-depth multidisciplinary collaborations with faculties and students from the Departments of Biological Sciences, Mechanical Engineering, Chemical Engineering and Orthopaedic Surgery using with state-of-the-art biological and engineering tools to perform research aimed atthe study of physical effects (e.g., cell deformation, fluid flow effects,hydrostatic pressure) on cells and tissues, and the incorporation of these forces in strategies to develop functional tissue substitutes of clinical relevance. An understanding of the effects of physical forces on cells isimportant in the development of effective tissue replacements which mimic orrestore normal tissue structure-function in orthopaedic and other load-bearing tissues of the body. Such studies are aim at alleviating the most prevalent and chronic problems afflicting the musculoskeletal system such as arthritis, and problems related to sports and occupational injuries.

Selected Publications

• Hung CT, Henshaw DR, Wang CC-B, Mauck R, Raia F, Palmer G, Mow VC, Ratcliffe A, Valhmu WB: Mitogen-activated protein kinase signaling in bovine articular chondrocytes in response to fluid flow does not require calcium mobilization. J Biomechanics 33:73-80, 2000.
• Chao P-HG, Roy R, Mauck RL, Liu W, Valhmu WB, Hung CT: Chondrocyte translocation response to direct current electric fields. J Biomech. Eng. 122:261-267, 2000.
• Palmer GD, Chao P-HG, Raia F, Mauck RL, Valhmu WB, Hung CT: Time dependent aggrecan gene expression of articular chondrocytes subjected to hyperosmotic loading. Osteoarthritis Cartilage: 9(8):761-770, 2001.
• Wang CC-B, Guo, XE, Sun, D, Mow VC, Ateshian GA, Hung CT: The functional environment of chondrocytes within cartilage subjected to compressive loading: theoretical and experimental approach. Biorheology, 39(1-2):39-45, 2002.
• Mauck RL, Wang CC-B, Oswald ES, Ateshian GA, Hung CT: The role of cell seeding density and nutrient supply for articular cartilage tissue engineering with deformational loading Osteoarthritis Cartilage11(12): 879-890, 2003.
• Lima EG, Mauck RL, Han S, Park S, Ng KW, Ateshian GA, Hung CT: Functional tissue engineering of chondral and osteochondral constructs. Biorheology41, 577-590, 2004.
• Ng KW, Wang CC-B, Mauck RL, Kelly TN, Chahine NO, Costa KD, Ateshian GA, Hung CT: A layered agarose approach to fabricate depth-dependent inhomogeneity in chondrocyte-seeded constructs.  J Orthop Res 23(1), 134-141,2005.
• Chao PHG, Tang Z, Angelini E, West AC, Costa KD, Hung CT: Dynamic osmotic loading of cells using a novel microfluidic device.  J Biomechanics 38(6), 1273-1281, 2005.
• Kelly TN, Ng KW, Wang CCB, Ateshian GA, Hung CT: Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures. J Biomechanics39(8):1489-1497, 2006.
• Ateshian GA, Likhitpanichkul M, Hung CT: A mixture theory analysis of passive transport in osmotic loading of cells.  J Biomechanics39(3):464-75, 2006
• Chahine NO, Ateshian GA, Hung CT: The effect of finite compressive strain on chondrocyte viability in statically loaded bovine articular cartilage. Biomech Model Mechanobiol6(1-2):103-111, 2007
•  Lima EG, Bian L, Ng KW, Mauck RL, Byers BA, Tuan RS, Ateshian GA, Hung CT: The effect of applied compressive loading on tissue-engineered cartilage constructs cultured with TGF-b3. Osteoarthritis Cartilage, 15(9): 1025-1033, 2007. 
• Chao PG, Lu HH, Hung CT*, Nicoll SB, Bulinski JC: Effects of applied DC electric field on ligament fibroblast migration and wound healing.  Connect Tissue Res, 48(4):188-197, 2007. (*corresponding author)
• Lima EG, Chao PG, Ateshian GA, Bal S, Cook JL, Vunjak-Novakovic G, Hung CT: The effect of devitalized trabecular bone on the formation of osteochondral tissue-engineered constructs, Biomaterials 29(32):4292-4299, 2008.
• Oswald ES, Chao PG, Bulinski JC, Ateshian GA, Hung CT: Zonal variation in chondrocyte water transport properties and proteoglycan production in juvenile bovine cartilage do not depend on osmotic environment.  Cell Mol Bioeng 1(4):339-348, 2008.
• Ng KW, Kugler LE, Doty SB, Ateshian GA, Hung CT: Scaffold degradation elevates the collagen content and dynamic compressive modulus in engineered articular cartilage.  Osteoarthritis Cartilage 17(2):220-227, 2009.
• Bian L, Angione SL, Ng KW, Williams DY, Mao DQ, Ateshian GA, Hung CT: Influence of decreasing nutrient path length on the development of engineered cartilage, Osteoarthritis Cartilage, 17(5):677-685, 2009.
• Bian L, Crivello K, Ng KW, Xu D, Williams DY, Ateshian GA, Hung CT: Influence of temporary chondroitinase ABC-induced GAG suppression on maturation of tissue engineered cartilage. Tissue Engineering 15(8):2065-2072, 2009.
• Ng KW, Lima EG, Bian L, O’Conor CJ, Jayabalan PS, Stoker AM, Kuroki K, Cook CR, Ateshian GA, Cook JL, Hung CT: Passaged adult chondrocytes can form engineered cartilage with functional mechanical properties: A canine model. Tissue Engineering, 16(3),1041-1051, 2010.
• Bian L, Fong JV, Lima EG, Stoker AM, Ateshian GA, Cook JL, Hung CT: Dynamic mechanical loading enhances functional properties of tissue engineered cartilage using mature canine chondrocytes. Tissue Engineering,16(5), 1781-1790, 2010.

Courses Taught

• BMEN 4501, 4502: Tissue Engineering I and II
• BMEN 3820: Biomedical Engineering Laboratory II
• Biomedical Engineering: Physical Effects on Cells (Summer High School Course in July)
  

Effects of physical, mechanical, and chemical stimuli on musculoskeletal cells related to cellular and tissue engineering.